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Biochim Biophys Acta. 2014 Mar;1842(3):402-13. doi: 10.1016/j.bbadis.2013.05.019. Epub 2013 May 22.

New methodologies for studying lipid synthesis and turnover: looking backwards to enable moving forwards.

Author information

  • 1Molecular Biomarkers, Merck, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. Electronic address: stephen_previs@merck.com.
  • 2Molecular Biomarkers, Merck, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA.

Abstract

Our ability to understand the pathogenesis of problems surrounding lipid accretion requires attention towards quantifying lipid kinetics. In addition, studies of metabolic flux should also help unravel mechanisms that lead to imbalances in inter-organ lipid trafficking which contribute to dyslipidemia and/or peripheral lipid accumulation (e.g. hepatic fat deposits). This review aims to outline the development and use of novel methods for studying lipid kinetics in vivo. Although our focus is directed towards some of the approaches that are currently reported in the literature, we include a discussion of the older literature in order to put "new" methods in better perspective and inform readers of valuable historical research. Presumably, future advances in understanding lipid dynamics will benefit from a careful consideration of the past efforts, where possible we have tried to identify seminal papers or those that provide clear data to emphasize essential points. This article is part of a Special Issue entitled: Modulation of Adipose Tissue in Health and Disease.

Copyright © 2013 Elsevier B.V. All rights reserved.

KEYWORDS:

Cardiovascular disease; Dyslipidemia; Kinetic biomarker; Mass spectrometry; Stable isotopes

PMID:
23707557
[PubMed - indexed for MEDLINE]
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