The effectiveness of detecting deception by measuring eye movements without making contact was investigated. A deception-detection protocol was used with two conditions based on the stimulus similarity to the critical image. Volunteer participants (N = 63) were randomly assigned to either the high or the low-similarity condition. They were randomly presented with critical and non-critical images and their eye movements were measured without contact. High-similarity images were presented in the high-similarity condition and low-similarity images were presented in the low-similarity condition. The results showed no significant differences in eye movements between the critical and non-critical images in the high-similarity condition. In contrast, in the low-similarity condition, the frequency and total length of time for glancing at critical images were significantly lower than for glancing at non-critical images. These results are suggestive of the effectiveness of the non-contact measurement of eye movements for the detection of deception.