RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing

PLoS One. 2013 May 17;8(5):e63960. doi: 10.1371/journal.pone.0063960. Print 2013.

Abstract

We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by comparing two honeybee and several stingless bee samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bees / genetics
  • Genotype
  • High-Throughput Nucleotide Sequencing / methods*
  • Metagenomics / methods*
  • Polymorphism, Single Nucleotide / genetics
  • Restriction Mapping / methods*

Grants and funding

European Union’s Seventh Framework Programme (grant agreement BEE DOC FP7-KBBE-2009-3 244956 CP-FP), Bundesministerium für Ernährung, Landwirtschaft und Verbraucherschutz (project FITBEE). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.