Characteristics of induced human pluripotent stem cells using DNA microarray technology

S P Medvedev; M A Smetanina; A I Shevchenko; I S Zakharova; A A Malakhova; E V Grigor'eva; E V Dementyeva; M A Aleksandrova; R A Poltavtseva; V N Veriasov; M L Filipenko; G T Sukhikh; E A Pokushalov; S M Zakian

doi:10.1007/s10517-013-2096-2

Characteristics of induced human pluripotent stem cells using DNA microarray technology

Bull Exp Biol Med. 2013 May;155(1):122-8. doi: 10.1007/s10517-013-2096-2.

Authors

S P Medvedev¹, M A Smetanina, A I Shevchenko, I S Zakharova, A A Malakhova, E V Grigor'eva, E V Dementyeva, M A Aleksandrova, R A Poltavtseva, V N Veriasov, M L Filipenko, G T Sukhikh, E A Pokushalov, S M Zakian

Affiliation

¹ Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

PMID: 23667889
DOI: 10.1007/s10517-013-2096-2

Abstract

We performed transcriptome analysis of some human induced pluripotent stem cells, embryonic stem cells, and human somatic cells using DNA microarrays. PluriTest bioinformatic system was used for evaluation of cell pluripotency. Changes in the genome structure and status of X-chromosome gene expression was analyzed using microarray technology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
DNA / genetics
Embryonic Stem Cells / cytology
Embryonic Stem Cells / physiology*
Gene Expression
Gene Expression Profiling
Genes, X-Linked*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / physiology*
Oligonucleotide Array Sequence Analysis
Transcriptome*

Substances

DNA