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Appl Environ Microbiol. 2013 Jul;79(14):4503-8. doi: 10.1128/AEM.00866-13. Epub 2013 May 10.

Zirex: a novel zinc-regulated expression system for Lactococcus lactis.

Author information

  • 1Department of Molecular Genetics, University of Groningen, Linnaeusborg, Groningen, The Netherlands.

Abstract

Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

PMID:
23666339
[PubMed - indexed for MEDLINE]
PMCID:
PMC3697519
Free PMC Article
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