Format

Send to:

Choose Destination
See comment in PubMed Commons below
Inorg Chem. 2013 Nov 4;52(21):12216-22. doi: 10.1021/ic400404g. Epub 2013 May 6.

Imaging DNA with fluorochrome bearing metals.

Author information

  • 1Center for Translational Nuclear Medicine and Molecular Imaging, ‡Martinos Center for Biomedical Imaging, Department of Radiology, and §Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School , Boston, Massachusetts 02114, United States.

Abstract

Molecules that fluoresce upon binding DNA are widely used in assaying and visualizing DNA in cells and tissues. However, using light to visualize DNA in animals is limited by the attenuation of light transmission by biological tissues. Moreover, it is now clear that DNA is an important mediator of dead cell clearance, coagulation reactions, and an immunogen in autoimmune lupus. Attaching metals (e.g., superparamagnetic nanoparticles, gadolinium ions, radioactive metal ions) to DNA-binding fluorochromes provides a way of imaging DNA in whole animals, and potentially humans, without light. Imaging metal-bearing, DNA-binding fluorochromes and their target DNA by magnetic resonance imaging may shed light on the many key roles of DNA in health and disease beyond the storage of genetic information.

PMID:
23646914
[PubMed - indexed for MEDLINE]
PMCID:
PMC3818342
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk