[Comparison of two nucleic acid extraction methods for norovirus in oysters]

Nan Fang Yi Ke Da Xue Xue Bao. 2013 Apr;33(4):568-72.
[Article in Chinese]

Abstract

Objective: To explore a convenient and effective method for norovirus nucleic acid extraction from oysters suitable for long-term viral surveillance.

Methods: Two methods, namely method A (glycine washing and polyethylene glycol precipitation of the virus followed by silica gel centrifugal column) and method B (protease K digestion followed by application of paramagnetic silicon) were compared for their performance in norovirus nucleic acid extraction from oysters. Real-time RT-PCR was used to detect norovirus in naturally infected oysters and in oysters with induced infection.

Results: The two methods yielded comparable positive detection rates for the samples, but the recovery rate of the virus was higher with method B than with method A.

Conclusion: Method B is a more convenient and rapid method for norovirus nucleic acid extraction from oysters and suitable for long-term surveillance of norovirus.

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centrifugation / methods*
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Ostreidae / virology*
  • RNA, Viral / isolation & purification*

Substances

  • RNA, Viral