Format

Send to:

Choose Destination
See comment in PubMed Commons below
Bioanalysis. 2013 May;5(9):1131-5. doi: 10.4155/bio.13.58.

A novel approach to capillary plasma microsampling for quantitative bioanalysis.

Author information

  • 1Bioanalytical Science & Toxicokinetics, DMPK, Platform Technology & Science, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA. chester.l.bowen@gsk.com

Abstract

BACKGROUND:

A novel device and procedure for the collection and isolation of microvolumes of plasma have been developed and two pilot rodent PK studies have been completed.

RESULTS:

This method involves collection of blood into a plastic-wrapped, EDTA-coated capillary tube, containing a small amount of a thixotropic gel and a porous plug. Following blood collection, the capillary is placed into a secondary labeled container suitable for centrifugation and plasma is generated. During centrifugation, the thixotropic gel isolates the plasma from the red blood cells and creates a physical barrier between the two matrices. The plasma is then dispensed from the capillary tube into a separate container for storage or processing.

CONCLUSION:

A simple and robust novel approach for the collection of small plasma volumes from rodent TK studies has been demonstrated.

PMID:
23641701
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Write to the Help Desk