Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genomics. 2013 Jul;102(1):38-46. doi: 10.1016/j.ygeno.2013.04.014. Epub 2013 Apr 29.

Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA.

Author information

  • 1Cancer and Disease Epigenetics Group, Murdoch Childrens Research Institute, Department of Paediatrics, The University of Melbourne, Royal Children's Hospital, Parkville, Victoria 3052, Australia. nwon@unimelb.edu.au

Abstract

Illumina Infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference mouse genome (mm9). We demonstrate reproducible measurements of DNA methylation at these probes in a range of mouse tissue samples and in a murine cell line model of acute myeloid leukaemia. In the absence of a mouse counterpart, the Infinium Human Methylation BeadChip arrays have utility for methylation profiling in non-human species.

Copyright © 2013 Elsevier Inc. All rights reserved.

PMID:
23639479
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk