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Sheng Wu Gong Cheng Xue Bao. 2013 Jan;29(1):119-22.

[Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis].

[Article in Chinese]

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  • 1Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.

Abstract

Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.

PMID:
23631126
[PubMed - indexed for MEDLINE]
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