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Bioorg Med Chem. 2013 Jun 1;21(11):2832-42. doi: 10.1016/j.bmc.2013.04.008. Epub 2013 Apr 17.

Sugar-attached upconversion lanthanide nanoparticles: a novel tool for high-throughput lectin assay.

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  • 1Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J2-10, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.


To create a novel high-throughput lectin assay (HTPLA) method based on the emission of a luminophore by highly penetrable near-infrared excitation, sugar-attached upconversion lanthanide nanoparticles (LNPs) were synthesized as a tool to highlight the aggregates caused by the sugar-mediated specific bridging between LNP and lectin. The emissions from a mannose-coated LNP in the aggregates with a mannose-binding lectin were much stronger than those from the non-aggregated samples, being sensitive enough for HTPLA. A galactose-coated LNP was also applicable to a macrophage aggregation assay for the sugar specificity of its surface lectin.

Copyright © 2013 Elsevier Ltd. All rights reserved.

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