Format

Send to:

Choose Destination
See comment in PubMed Commons below
Bioorg Med Chem. 2013 Jun 1;21(11):2832-42. doi: 10.1016/j.bmc.2013.04.008. Epub 2013 Apr 17.

Sugar-attached upconversion lanthanide nanoparticles: a novel tool for high-throughput lectin assay.

Author information

  • 1Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J2-10, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

Abstract

To create a novel high-throughput lectin assay (HTPLA) method based on the emission of a luminophore by highly penetrable near-infrared excitation, sugar-attached upconversion lanthanide nanoparticles (LNPs) were synthesized as a tool to highlight the aggregates caused by the sugar-mediated specific bridging between LNP and lectin. The emissions from a mannose-coated LNP in the aggregates with a mannose-binding lectin were much stronger than those from the non-aggregated samples, being sensitive enough for HTPLA. A galactose-coated LNP was also applicable to a macrophage aggregation assay for the sugar specificity of its surface lectin.

Copyright © 2013 Elsevier Ltd. All rights reserved.

PMID:
23628472
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk