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Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3812-4.

[Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa].

[Article in Chinese]

Author information

  • 1Key Laboratory of Plant Resources and Biology of Anhui Province, School of Life Science, Huaibei Normal University, Huaibei 235000, China. xuejp2000@yahoo.com.cn

Abstract

OBJECTIVE:

To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.

METHOD:

Leaves from test-tube seedlings of 85-5 R. glutinosa were used as explants. After rooting induction, they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R. glutinosa.

RESULT AND CONCLUSION:

NAA played a significant role in induction of test-tube tuberous roots of R. glutinosa, followed by sucrose and 6-BA. With leaves from test-tube seedlings as the explants, the optimal medium for inducing test-tube tuberous roots of R. glutinosa was MS + BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 7%. The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R. glutinosa.

PMID:
23627185
[PubMed - indexed for MEDLINE]
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