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Exp Toxicol Pathol. 2013 Nov;65(7-8):1043-51. doi: 10.1016/j.etp.2013.03.003. Epub 2013 Apr 26.

Ultrastructural changes in the air-blood barrier in mice after intratracheal instillations of Asian sand dust and gold nanoparticles.

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  • 1Department of Veterinary Pathology, Tottori University, Minami 4-101, Koyama, Tottori-shi, Tottori 680-8553, Japan; The United Graduated School of Veterinary Science, Yamaguchi University, Yoshida 1667-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan.


The purpose of this study was to investigate a possible translocation pathway of intratracheally instilled gold nanoparticles after the induction of acute pulmonary injury by Asian sand dust. ICR mice were intratracheally instilled with 800μg Asian sand particles (CJ-2 particles) 24h before instillation of 50-nm gold nanoparticles. Lungs from mice treated with Asian sand particles and gold nanoparticles showed an acute focal inflammation with an increased expression of proinflammatory cytokines (IL-6 and TNF-α) and oxidative stress markers (Cu/Zn SOD and iNOS) in alveolar macrophages, type I alveolar epithelial cells, and endothelial cells at the alveolar walls. Electron microscopy revealed a destruction of the alveolar walls with an increased number of endocytic vesicles in the cytoplasm of both type I epithelial cells and endothelial cells; gold nanoparticles were demonstrated in these endocytic vesicles. These findings suggest that translocation of the exposed nanoparticles may be enhanced in the lung tissues with acute inflammatory changes.

Copyright © 2013 Elsevier GmbH. All rights reserved.


Air–blood barrier; Cu/Zn SOD; IL-6; Kosa; Laminin; TNF-α; Translocation pathway; iNOS

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