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Asian Pac J Trop Biomed. 2013 Mar;3(3):217-22. doi: 10.1016/S2221-1691(13)60053-2.

Hepatitis B virus X protein up-regulates tumor necrosis factor-α expression in cultured mesangial cells via ERKs and NF-κB pathways.

Author information

  • 1Deptartment of Pediatrics, Clinical Medical College, Yangtze University, Jingzhou 434000, China.

Abstract

OBJECTIVE:

To investigate the effects of hepatitis B virus (HBV) X protein (HBx) on the expression of tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (GMCs) and the underlying intracellular signal pathways.

METHODS:

The plasmid pCI-neo-X that carries the X gene of hepatitis B virus was transfected into cultured GMCs. HBx expression in the transfected GMCs was assessed by Western-blot. TNF-α protein and mRNA were assessed by ELISA and semi-quantitative RT-PCR, respectively. Three kinase inhibitors-U0126, an inhibitor of extracellular signal-regulated kinases (ERKs); lactacystin, an inhibitor of nuclear factor-κB (NF-κB); and SB203580, a selective inhibitor of p38 MAP kinase (p38 MAPK) were used to determine which intracellular signal pathways may underlie the action of HBx on TNF-α expression in transfected GMCs.

RESULTS:

A significant increase in HBx expression in pCI-neo-X transfected GMCs was detected at 36 h and 48 h, which was not affected by any of those kinase inhibitors mentioned above. A similar increase in the expression of both TNF-α protein and mRNA was also observed at 36 h and 48 h, which was significantly decreased in the presence of U0126 or lactacytin, but not SB203580.

CONCLUSIONS:

HBx upregulates TNF-α expression in cultured GMCs, possibly through ERKs and NF-κB pathway, but not p38 MAPK pathway.

KEYWORDS:

Extracellular signal-regulated kinase; Glomerulonephritis; Heptitis B virus; Nuclear factor-κB; Tumor necrosis factor-α; X protein

PMID:
23620841
[PubMed - in process]
PMCID:
PMC3631753
Free PMC Article

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