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Vet Comp Oncol. 2013 Apr 24. doi: 10.1111/vco.12035. [Epub ahead of print]

Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours.

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  • 1Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan; Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt.

Abstract

We examined whether mutation of the platelet-derived growth factor receptor protein tyrosine kinase (PDGFR)-α and PDGFR-β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans- or juxtamembrane regions of PDGFR-α and PDGFR-β were analysed with immunohistochemical staining and polymerase chain reaction-direct sequencing using DNA from paraffin-embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR-α and almost most of the HA cases were negative for PDGFR-β. Of the HSA cases, 55.6% were negative for PDGFR-α and 63% were strongly positive for PDGFR-β. Among the HA cases, 1 missense mutation was detected in PDGFR-α exon 18 and 1 in PDGFR-β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR-β. Thus, genomic mutation of trans- or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.

© 2013 Blackwell Publishing Ltd.

KEYWORDS:

PDGFR; dog; hemangioma; hemangiosarcoma; immunohistochemistry; mutation

PMID:
23611531
[PubMed - as supplied by publisher]
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