Format

Send to:

Choose Destination
See comment in PubMed Commons below
Gene. 2013 Jul 10;523(2):161-6. doi: 10.1016/j.gene.2013.04.002. Epub 2013 Apr 18.

The 16S rRNA and mcrA gene based comparative diversity of methanogens in cattle fed on high fibre based diet.

Author information

  • 1Nutrition Biotechnology Lab, Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India. nutritionbiotechnology@gmail.com

Abstract

In the present study, the diversity of rumen methanogens in crossbred Karan Fries cattle was determined by constructing 16S rRNA and mcrA (methyl coenzyme-M reductase α subunit) gene libraries using specific primers. All thirteen OTUs or phylotypes from 16S rRNA library clustered with order Methanobacteriales, twelve of which aligned with Methanobrevibacter spp., whereas one OTU resemble with Methanosphaera stadtmanae. Out of eighteen OTUs identified from mcrA gene library, fifteen clustered with order Methanobacteriales, two resemble with Methanomicrobiales and remaining one grouped with Methanosarcinales. These results revealed that Methanobrevibacter phylotype was predominantly present in Karan Fries crossbred cattle fed on high fibrous diet containing wheat straw. Compared to 16S rRNA gene, mcrA gene OTUs clustered in three orders providing better insights of rumen methanogens diversity in cattle.

Copyright © 2013 Elsevier B.V. All rights reserved.

PMID:
23603353
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk