Send to

Choose Destination
See comment in PubMed Commons below
Environ Health Perspect. 2013 Jun;121(6):717-24. doi: 10.1289/ehp.1206301. Epub 2013 Apr 19.

A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.

Author information

  • 1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 , USA.



Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.


We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.


We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database.


The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression.


This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for National Institute of Environmental Health Sciences Icon for PubMed Central
    Loading ...
    Write to the Help Desk