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Clin Nutr. 2014 Feb;33(1):130-5. doi: 10.1016/j.clnu.2013.03.012. Epub 2013 Mar 26.

Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.

Author information

  • 1Clermont Université, Université d'Auvergne, UMR 1019, Unité de Nutrition Humaine, CRNH-Auvergne, CLARA, BP 10448, F-63000 Clermont-Ferrand, France.
  • 2Centre de recherche INRA, UMR 1019 INRA Université d'Auvergne, Unité de Nutrition Humaine, Clermont-Ferrand Theix, France.
  • 3CHU Clermont-Ferrand, Centre de Pharmacologie Clinique, Inserm, CIC 501, Univ Clermont 1, Faculté de Médecine, F-63001 Clermont-Ferrand, France.
  • 4Centre anticancéreux Jean-Perrin, CHU, Unité de Nutrition, CLARA, F-63000 Clermont-Ferrand, France. Electronic address: m-paule.vasson@udamail.fr.

Abstract

BACKGROUND & AIMS:

Polyphenols exert a variety of biological properties, including antioxidant, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men, we investigated the effects of orange juice and its major polyphenolic compound hesperidin on a panel of immune cell functions, including cytokine secretion by leukocytes, lytic activity of NK cells, and the Reactive Oxygen Species (ROS) burst by polymorphonuclear neutrophil cells (PMN).

METHODS:

The protocol design was divided into three 4-week treatment periods separated by 3-week wash-out intervals, for total study duration of 18 weeks. During treatment periods, volunteers (n = 24) consumed daily 500 mL of orange juice, or an isocaloric control beverage with hesperidin (292 mg in a capsule), or of the same control beverage with a placebo.

RESULTS:

Whatever the intake was, no variations were recorded on leukocyte subset distributions (PMN, B and T lymphocytes, NK cells and monocytes), ROS production by stimulated PMNs, lytic activity of NK cells or cytokine production capacity of leukocytes in well-nourished healthy volunteers.

CONCLUSIONS:

We show that consumption within the usual daily intake range of orange juice and its major polyphenol hesperidin do not induce immunomodulation of cell immune function in healthy well-nourished humans.

Copyright © 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

KEYWORDS:

Anti-oxidant status; Cytokines; ECD; EGFP; Enhanced Green Fluorescent Protein; FITC; Fluorescein IsothioCyanate; Leukocyte functions; NK; NK cells; Natural Killer; Oxidative response; PBMC; PC5; PE; PE-Cyanine 5; PE-Texas red; PHA; PMA; PMN; Peripheral Blood Mononuclear Cells; Phorbol Myristate Acetate; PhycoErythrine; PhytoHemAgglutinin; PolyMorphoNuclear neutrophil cells; ROS; Reactive Oxygen Species

PMID:
23602614
[PubMed - indexed for MEDLINE]
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