Unusually high rates of metabolism of DHT in cytosols of the quail uropygial gland

Y Amet; S di Stefano; E Quemener; J H Abalain; H H Floch; J Y Daniel

doi:10.1016/0039-128x(90)90020-c

Unusually high rates of metabolism of DHT in cytosols of the quail uropygial gland

Steroids. 1990 May;55(5):228-32. doi: 10.1016/0039-128x(90)90020-c.

Authors

Y Amet¹, S di Stefano, E Quemener, J H Abalain, H H Floch, J Y Daniel

Affiliation

¹ Laboratoire de Biochimie, UA 598 du CNRS, Faculté de Médecine, Brest, France.

PMID: 2360219
DOI: 10.1016/0039-128x(90)90020-c

Abstract

The metabolism of DHT in the cytosol of the quail uropygial gland was found to be so high that the steroid was almost completely inactivated within 2 hours of incubation at 0 C. In these conditions, DHT cannot be used for the characterization of androgen receptors. By contrast, R 1881 and mibolerone, which are not metabolized, can be used as alternative ligands. Moreover, the extremely high metabolism of DHT questions the physiologic role of this steroid in the quail uropygial gland.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytosol / metabolism*
Dihydrotestosterone / metabolism*
Male
Metribolone / metabolism
Nandrolone / analogs & derivatives
Nandrolone / metabolism
Quail / metabolism*
Receptors, Androgen / metabolism
Sebaceous Glands / metabolism*
Testosterone / metabolism
Testosterone Congeners / metabolism

Substances

Receptors, Androgen
Testosterone Congeners
Dihydrotestosterone
Metribolone
Testosterone
Nandrolone
mibolerone