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Analyst. 2013 Jun 7;138(11):3135-8. doi: 10.1039/c3an00170a.

Nanotips for single-step preparation of DNA for qPCR analysis.

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  • 1Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.


A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

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