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PLoS One. 2013 Apr 10;8(4):e60988. doi: 10.1371/journal.pone.0060988. Print 2013.

Single cell analysis of drug distribution by intravital imaging.

Author information

  • 1Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America.

Abstract

Recent advances in the field of intravital imaging have for the first time allowed us to conduct pharmacokinetic and pharmacodynamic studies at the single cell level in live animal models. Due to these advances, there is now a critical need for automated analysis of pharmacokinetic data. To address this, we began by surveying common thresholding methods to determine which would be most appropriate for identifying fluorescently labeled drugs in intravital imaging. We then developed a segmentation algorithm that allows semi-automated analysis of pharmacokinetic data at the single cell level. Ultimately, we were able to show that drug concentrations can indeed be extracted from serial intravital imaging in an automated fashion. We believe that the application of this algorithm will be of value to the analysis of intravital microscopy imaging particularly when imaging drug action at the single cell level.

PMID:
23593370
[PubMed - indexed for MEDLINE]
PMCID:
PMC3622689
Free PMC Article

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