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Am J Reprod Immunol. 2013 Sep;70(3):213-20. doi: 10.1111/aji.12118. Epub 2013 Apr 17.

Magnesium sulfate increases intracellular magnesium reducing inflammatory cytokine release in neonates.

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  • 1Department of Reproductive Biology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

Abstract

PROBLEM:

Magnesium sulfate (MgSO4 ) exposure reduces the risk of cerebral palsy. As neonatal inflammatory cytokine levels strongly correlate with neurologic outcome, we hypothesize that MgSO4 decreases inflammatory cytokine production.

METHOD OF STUDY:

We assessed the effect of MgSO4 on cellular magnesium levels, cytokine production, and release within THP-1 and cord blood mononuclear cells.

RESULTS:

MgSO4 exposure increased intracellular magnesium levels, reducing the frequency of THP-1 cells producing IL-1β, IL-8, and TNF-α following LPS stimulation. Significant reductions in the frequency of neonatal monocytes producing TNF-α (48%) and IL-6 (37%) were seen following LPS stimulation, and MgSO4 also significantly decreased the frequency of monocytes producing TNF-α (35%) under basal conditions. Decreased cytokine production was confirmed via ELISA, demonstrating a sustained effect and dose response. Magnesium also reduced cytokine production following stimulation with TLR ligands representing obstetrical infections (group B Streptococcus and Mycoplasma) and in preterm neonatal monocytes.

CONCLUSION:

MgSO4 increases intracellular magnesium, reducing inflammatory cytokine production and release, potentially elucidating the mechanism by which MgSO4 prevents cerebral palsy, eclampsia, and preterm birth.

© 2013 John Wiley & Sons Ltd.

KEYWORDS:

IL-6; TNF-α; inflammation; magnesium

PMID:
23590581
[PubMed - indexed for MEDLINE]
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