(**A** and **B**) Representative cryosections (**A**) and relative density (**B**) of untreated (from Figure D) and PTX-treated (*n* = 358) fetal DP thymocytes on CD11c-YFP slices. (**C** and **D**) Average speed of (**C**) untreated (from Figure B) or PTX-treated (*n* = 80) fetal DP cells in the cortex of CD11c-YFP slices and on (**D**) human fetal thymic slices. *n* = 97 (DP); 73 (DP+PTX). (**E** and **F**) Fetal DP thymocytes were incubated with AMD3100 at the start of culture, and their location was determined after 4 hours. Representative cryosections (**E**) and relative density (**F**) of untreated (from Figure D) or AMD3100-treated (*n* = 2,358) fetal DP thymocytes on CD11c-YFP slices. (**G** and **H**) Fetal DP thymocytes were overlaid on CD11c-YFP slices for 4 hours, washed, then treated with AMD3100 for another 4 hours. Representative cryosections (**G**) and relative density (**H**) of untreated (*n* = 5,282) or AMD3100-treated (*n* = 1,530) fetal DP thymocytes on CD11c-YFP slices. (**I**) Postnatal (PN) DP thymocytes were overlaid onto CD11c-YFP or human postnatal slices for 4 hours, washed, then treated or not with AMD3100 for another 4 hours. *n* = 1,006 (mouse DP); 2,221 (mouse DP+AMD3100); 2,752 (postnatal human DP); 1,308 (postnatal human DP+AMD3100). (**J**) Relative density of untreated (*n* = 1,392) or AMD3100-treated (*n* = 1,605) fetal CD4^{+} and CD8^{+} SP thymocytes on CD11c-YFP slices. Data were compiled from at least 3 tissue sections from at least 2 independent experiments. (**A**, **E**, and **G**) Solid and dashed outlines as in Figure . (**C** and **D**) Symbols and lines as in Figure . (**B**, **F**, and **H**–**J**) Each dot represents quantification of 1 tissue section. Scale bars: 100 μm. **P* < 0.05.

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