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J Trace Elem Med Biol. 2013 Oct;27(4):326-33. doi: 10.1016/j.jtemb.2013.02.001. Epub 2013 Feb 18.

Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn2+ cations in cartilage and meniscus calcifications.

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  • 1Laboratoire de Physique des Solides, CNRS UMR 8502, Paris Sud University, Orsay, France.

Abstract

We aimed to examine the presence of Zn, a trace element, in osteoarthritis (OA) cartilage and meniscus from patients undergoing total knee joint replacement for primary OA. We mapped Ca(2+) and Zn(2+) at the mesoscopic scale by X-ray fluorescence microanalysis (μX-ray) to determine the spatial distribution of the 2 elements in cartilage, μX-ray absorption near edge structure spectroscopy to identify the Zn species, and μX-ray diffraction to determine the chemical nature of the calcification. Fourier transform infrared spectroscopy was used to determine the chemical composition of cartilage and meniscus. Ca(2+) showed a heterogeneous spatial distribution corresponding to the calcifications within cartilage (or meniscus) or at their surface. At least 2 Zn(2+) species were present: the first may correspond to Zn embedded in protein (different Zn metalloproteins are known to prevent calcification in biological tissues), and the second may be associated with a Zn trap in or at the surface of the calcification. Calcification present in OA cartilage may significantly modify the spatial distribution of Zn; part of the Zn may be trapped in the calcification and may alter the associated biological function of Zn metalloproteins.

Copyright © 2013 Elsevier GmbH. All rights reserved.

KEYWORDS:

Ca phosphate apatites; Ca pyrophosphate; Osteoarthritis; Zn environment; μX-ray absorption spectroscopy

PMID:
23582484
[PubMed - indexed for MEDLINE]
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