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Nucleic Acids Res. 2013 May 1;41(10):e112. doi: 10.1093/nar/gkt228. Epub 2013 Apr 10.

Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs.

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  • 1Population Genetics Technologies Ltd., Babraham Institute, Babraham, Cambridgeshire CB22 3AT, UK.

Abstract

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.

PMID:
23580546
[PubMed - indexed for MEDLINE]
PMCID:
PMC3664814
Free PMC Article

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