Sensory interaction between attractant diacetyl and repellent 2-nonanone in the nematode Caenorhabditis elegans

In the nematode Caenorhabditis elegans, the odorant diacetyl is sensed by AWA sensory neurons in the amphid sensory organ and elicits an attractive response, whereas 2-nonanone is sensed by AWB amphid sensory neurons and elicits an avoidance response. In the present study, we report that nematodes exhibit a sensory interaction between the attractant diacetyl and repellent 2-nonanone. In the presence of food, the chemotactic response to 0.01% diacetyl in nematodes preexposed to 0.1% diacetyl was greater than that in nonexposed naive nematodes (P < 0.05). The response to diacetyl was also greater in nematodes preexposed to 3% 2-nonanone in the presence of food than that in naive nematodes (P < 0.01). In the absence of food, the response to diacetyl in nematodes preexposed to diacetyl or 2-nonanone was significantly lower than that in nonexposed control nematodes (P < 0.01). The avoidance response to 10% 2-nonanone in nematodes preexposed to each odorant in the presence or absence of food was lower than that in nonexposed nematodes (P < 0.05). To confirm the validity of our results, the chemotactic responses to diacetyl and 2-nonanone were observed using che-3, odr-4, and odr-10 mutants, which exhibited defective sensitivity to diacetyl or 2-nonanone. From the results of our experiments, we conclude that nematodes exhibit a sensory interaction between diacetyl and 2-nonanone and speculate that this interaction is driven by higher-level neuronal circuits that underlie sensory integration.