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Fertil Steril. 2013 Jul;100(1):262-8.e1-2. doi: 10.1016/j.fertnstert.2013.03.021. Epub 2013 Apr 8.

Aging sperm negatively impacts in vivo and in vitro reproduction: a longitudinal murine study.

Author information

  • 1National Foundation for Fertility Research, Lone Tree, Colorado, USA. mkatz-jaffe@fertilityresearch.org

Abstract

OBJECTIVE:

To investigate the impact of paternal aging on reproductive success.

DESIGN:

Animal study.

SETTING:

Research facility.

ANIMAL(S):

Outbred CF1 mice.

INTERVENTION(S):

Ten young male mice with proven fertility were mated routinely over 15 months with superovulated young females to assess in vivo and in vitro reproductive outcome.

MAIN OUTCOME MEASURE(S):

In vivo fertilization, in vivo fetal development, in vitro embryo morphology, and developmental outcome were assessed.

RESULT(S):

There were no differences observed for any reproductive end point until the paternal age of 12 months. At 12-15 months, in vivo fertilization was significantly decreased (35% vs. 78% at <12 months). Natural matings with males ≥ 12 months revealed significantly smaller fetuses (11.36 mm vs. 14.73 mm <12 months) and placental weight (0.10 g vs. 0.13 g at <12 months). In vitro blastocyst development showed a significant decline at ≥ 12 months, and in vitro blastocyst transfer resulted in a significant increase in pregnancy loss with males ≥ 12 months (61.5% vs. 0% at <12 months). In addition, the expression levels of Ace-1, Prm1, Prm2, and Smcp were observed to be decreased in sperm from males ≥ 12 months compared with young male control subjects.

CONCLUSION(S):

Results from this study indicate an abrupt reproductive deterioration at paternal midlife, with an adverse effect observed on natural conception, in vitro blastocyst development, implantation potential, and fetal viability.

Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

PMID:
23579004
[PubMed - indexed for MEDLINE]
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