Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Biopolymers. 2014 Feb;101(2):180-7. doi: 10.1002/bip.22254.

Monitoring of post-translational modification dynamics with genetically encoded fluorescent reporters.

Author information

  • 1Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205.


Post-translational modifications (PTMs) of proteins are essential mechanisms for virtually all dynamic processes within cellular signaling networks. Genetically encoded reporters based on fluorescent proteins (FPs) are powerful tools for spatiotemporal visualization of cellular parameters. Consequently, commonly used modular biosensor designs have been adapted to generate several protein-based indicators for monitoring various PTMs or the activity of corresponding enzymes in living cells, providing new biological insights into dynamics and regulatory functions of individual PTMs. In this review, we describe the application of general design strategies focusing on PTMs and discuss important considerations for engineering feasible indicators depending on the purpose. Moreover, we present developments and enhancements of PTM biosensors from selected studies and give an outlook on future perspectives of this versatile approach. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 180-187, 2014.

Copyright © 2013 Wiley Periodicals, Inc.


FRET; biosensor; microscopy

[PubMed - in process]
[Available on 2015/2/1]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for John Wiley & Sons, Inc.
    Loading ...
    Write to the Help Desk