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Physiol Mol Biol Plants. 2011 Oct;17(4):375-85. doi: 10.1007/s12298-011-0079-1. Epub 2011 Aug 27.

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [ Cajanus cajan (L.) Millsp.] cv. JKR105.

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  • 1College of Biotechnology & Allied Sciences, Allahabad Agricultural Institute Deemed University, Allahabad, 211 007 UP India ; Biotechnology Division, JK Agri Genetics Ltd., 1-10-77, 4th Floor, Varun Towers, Begumpet, Hyderabad, 500 016 AP India.

Abstract

In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L(-1) 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L(-1) 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

KEYWORDS:

Auxins; Cytokinins; Organogenesis; Pigeon pea; Somatic embryogenesis

PMID:
23573031
[PubMed]
PMCID:
PMC3550589
Free PMC Article
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