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Int J Biol Macromol. 2013 Jul;58:179-85. doi: 10.1016/j.ijbiomac.2013.03.043. Epub 2013 Apr 6.

An anti-complementary polysaccharide from the roots of Bupleurum chinense.

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  • 1School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.

Abstract

Bioactivity-guided fractionation of hot-water extract from the roots of Bupleurum chinense DC led to the isolation of a homogeneous polysaccharide BC-PS1 composed of Gal, GalA, Glc, Ara and Man in the ratio of 1.6:1.1:1.8:1.7:1.0, along with trace of Rha, Fuc and Xyl. The main linkages of the residues of BC-PS1 include terminal, 1,4-linked and 1,3-linked Galp (GalA), terminal and 1,4-linked Glcp, terminal and 1,5-linked Araf, 1,4,6-linked Manp. Bioassays revealed that the concentrations of BC-PS1 that resulted in 50% hemolytic inhibition on classical complement pathway (CH50) and alternative complement pathway (AP50) were 0.199±0.025 mg/mL and 0.371±0.017 mg/mL, respectively. Preliminary mechanism study indicated that BC-PS1 interacted with C1q, C2, C5 and C9.

Copyright © 2013 Elsevier B.V. All rights reserved.

PMID:
23570674
[PubMed - indexed for MEDLINE]
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