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Rev Soc Bras Med Trop. 2013 Jan-Feb;46(1):97-9.

Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil.

Author information

  • 1Centro de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.

Abstract

INTRODUCTION:

West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil.

METHODS:

Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus.

RESULTS:

WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses.

CONCLUSIONS:

This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.

PMID:
23563834
[PubMed - indexed for MEDLINE]
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