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Biotechnol Adv. 2013 Nov;31(6):936-44. doi: 10.1016/j.biotechadv.2013.03.009. Epub 2013 Apr 4.

Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development: advances and applications.

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  • 1School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of Korea.


The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.

Copyright © 2013 Elsevier Inc. All rights reserved.


Biosensor; Carbohydrate binding module; Cell surface anchoring; Cellulosome; Clostridium cellulovorans; Cohesin–dockerin interaction; Consolidated bioprocessing; Designer minicellulosome; One-step CBM purification; Whole-cell biocatalyst

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