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Bioorg Med Chem Lett. 2013 May 1;23(9):2598-600. doi: 10.1016/j.bmcl.2013.02.106. Epub 2013 Mar 7.

Identification of metal ion binding peptides containing unnatural amino acids by phage display.

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  • 1Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.


The bidentate metal binding amino acid bipyridylalanine (BpyAla) was incorporated into a disulfide linked cyclic peptide phage displayed library to identify metal ion binding peptides. Selection against Ni(2+)-nitrilotriacetic acid (NTA) enriched for sequences containing histidine and BpyAla. BpyAla predominated when selections were carried out at lower pH, consistent with the differential pKa's of histidine and BpyAla. Two peptides containing BpyAla were synthesized and found to bind Ni(2+) with low micromolar dissociation constants. Incorporation of BpyAla and other metal binding amino acids into peptide and protein libraries should enable the evolution of novel binding and catalytic activities.

Copyright © 2013. Published by Elsevier Ltd.

[PubMed - indexed for MEDLINE]
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