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Asian Pac J Cancer Prev. 2013;14(1):507-10.

Construction and expression of an eukaryotic expression vector containing the IER3 gene.

Author information

  • 1Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.

Abstract

BACKGROUND:

More and more research indicate that the immediately early response gene 3 (IER3) is involved in many biological processes, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression.

METHODS:

Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western- blotting.

RESULTS:

Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls.

CONCLUSION:

The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.

PMID:
23534784
[PubMed - indexed for MEDLINE]
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