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J Clin Monit Comput. 2013 Oct;27(5):499-508. doi: 10.1007/s10877-013-9456-3. Epub 2013 Mar 26.

Factors affecting hemoglobin measurement.

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  • 1Department of Anesthesiology and Critical Care Medicine, Johns Hopkins School of Medicine, 600 N. Wolfe St., Meyer 8-134, Baltimore, MD, 21287, USA,


A review of the literature shows that current "standard" laboratory measurements for hemoglobin are subject to numerous factors that affect both accuracy and reliability. In addition, total hemoglobin concentration measurements are subject to numerous factors that affect the "true" hemoglobin value. This article discusses both the physiologic factors that influence hemoglobin levels and the technical aspects and variability among the different measurement methodologies currently available.

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