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Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24.

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.

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  • 1Department of Photobiology and Bioimaging, The Scintillon Institute, San Diego, California, USA. nathanshaner@scintillon.org

Abstract

We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

PMID:
23524392
[PubMed - indexed for MEDLINE]
PMCID:
PMC3811051
Free PMC Article
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