Send to:

Choose Destination
See comment in PubMed Commons below
Channels (Austin). 2013 May-Jun;7(3):160-70. doi: 10.4161/chan.24024. Epub 2013 Mar 19.

Efficient experimental design and analysis of real-time PCR assays.

Author information

  • 1Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.


Real-time polymerase chain reaction (qPCR) is currently the standard for gene quantification studies and has been extensively used in large-scale basic and clinical research. The operational costs and technical errors can become a significant issue due to the large number of sample reactions. In this paper, we present an experimental design strategy and an analysis procedure that are more efficient requiring fewer sample reactions than the traditional approach. We verified mathematically and experimentally the new design on a well-characterized model, to evaluate the gene expression levels of CACNA1C and CACNA1G in hypertrophic ventricular myocytes induced by phenylephrine treatment.


ANF; L-type calcium channel; Real-time PCR; T-type calcium channel; cell culture; gene expression; hypertrophy; ventricular myocytes

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Landes Bioscience Icon for PubMed Central
    Loading ...
    Write to the Help Desk