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Appl Biochem Biotechnol. 2013 May;170(2):301-19. doi: 10.1007/s12010-013-0155-9. Epub 2013 Mar 19.

Exon array analysis of alternative splicing of genes in SOD1G93A transgenic mice.

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  • 1Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China.

Abstract

Alternative splicing is a common strategy for creating functional diversities of proteins. While conventional identification of splice variants generally targets individual genes in amyotrophic lateral sclerosis, we present a novel exon-centric array that allows genome-wide identification of splice variants and concurrently provides analysis of gene expression. Compare 1 was asymptomatic SOD1G93A transgenic mice with nontransgenic littermates; compare 2 was symptomatic with asymptomatic transgenic mice. RT-PCR was performed to validate. Pathway and GO analysis were performed on abnormal genes. These findings could guide us to demonstrated the potential influence of mutant human CuZn-SOD1 and of splicing regulation in pathological processes.

PMID:
23508861
[PubMed - indexed for MEDLINE]
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