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Chin Med J (Engl). 2013 Mar;126(6):1138-43.

Reprogramming of adult human neural stem cells into induced pluripotent stem cells.

Author information

  • 1Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.

Abstract

BACKGROUND:

Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.

METHODS:

NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.

RESULTS:

Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.

CONCLUSION:

Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.

PMID:
23506594
[PubMed - indexed for MEDLINE]
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