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Dig Dis Sci. 2013 Jul;58(7):1863-70. doi: 10.1007/s10620-013-2612-2. Epub 2013 Mar 17.

MiR-21 down-regulation suppresses cell growth, invasion and induces cell apoptosis by targeting FASL, TIMP3, and RECK genes in esophageal carcinoma.

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  • 1College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. wnsky@zzu.edu.cn

Abstract

BACKGROUND:

miR-21 is overexpressed in esophageal squamous cell carcinoma (ESCC) and is thought to be correlated with the development of the cancer. The target gene of miR-21 including FASL, TIMP3 and RECK is revealed by researchers. miR-21 may be involved in the tumorgenesis of ESCC by targeting FASL, TIMP3 and RECK.

AIMS:

The purpose of this study was to explore the mechanism of miR-21 in the development of ESCC.

METHODS:

miR-21 expression in ESCC and the matched non-malignant adjacent tissues (NMATs) was examined by qRT-PCR. Cell growth, cell apoptosis and cell invasion ability of EC9706 and EC-1 cells was examined after the cells were transfected with miR-21 inhibitor. The potential target genes of miR-21 including FASL, TIMP3 and RECK were examined by western blot and Luciferase reporter assay.

RESULTS:

miR-21 expression was increased significantly in ESCC tissues compared with NMAT. miR-21 down-regulation inhibits cell growth, cell invasion and induces cells to apoptosis. FASL, TIMP3 and RECK are direct targets of miR-21.

CONCLUSIONS:

miR-21 down-regulation inhibits cell growth, invasion and induces cells to apoptosis by targeting FASL, TIMP3 and RECK genes.

[PubMed - indexed for MEDLINE]
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