Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of peptide citrullination site using Br signature

Anal Biochem. 2013 Jun 1;437(1):62-7. doi: 10.1016/j.ab.2013.03.003. Epub 2013 Mar 13.

Abstract

Enzymatic conversion of arginine to citrulline by peptidyl arginine deiminase is associated with peptide presentation and development of autoimmunity in rheumatoid arthritis. In order to facilitate identification of the citrullination site, citrulline residue was modified using 4-bromophenyl glyoxal, and 194Da mass increase and incorporation of the Br signature were confirmed by MALDI-TOF MS. Using this approach, we identified four and five citrullination sites of bovine serum albumin and bovine fibrinogen, respectively. MALDI-TOF/TOF MS was used to unambiguously identify two citrullination sites from bovine fibrinogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arginine / metabolism
  • Binding Sites
  • Bromine / chemistry*
  • Cattle
  • Citrulline / metabolism*
  • Fibrinogen / chemistry
  • Fibrinogen / metabolism
  • Glyoxal / chemistry
  • Peptides / chemistry
  • Peptides / metabolism*
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Peptides
  • Serum Albumin, Bovine
  • Citrulline
  • Glyoxal
  • Fibrinogen
  • Arginine
  • Bromine