Innovative microwave-assisted hydrolysis of ellagitannins and quantification as ellagic acid equivalents

Food Chem. 2013 Jun 15;138(4):2430-4. doi: 10.1016/j.foodchem.2012.12.015. Epub 2012 Dec 28.

Abstract

The health-promoting effects of ellagic acid and its intestinal degradation products are well-known. In plants, ellagic acid mainly appears in the form of its precursors, the so-called ellagitannins. Therefore, determination of total ellagic acid content has been accomplished by cleaving ellagitannins with high temperatures and strong acids. Microwave-assisted extraction (MAE) has been shown to be a very effective and quick extraction technique. The aim of the present study was to establish an MAE-based method for a rapid hydrolysis of ellagitannins and to compare this method with a conventional acid hydrolysis. For this purpose, strawberries (Fragaria x ananassa), which have been identified as a major source of ellagitannins in human diet, were used as a model. The newly developed MAE method was quicker, less chemical-consuming and more effective in hydrolysing ellagitannins.

Publication types

  • Evaluation Study

MeSH terms

  • Analytic Sample Preparation Methods / instrumentation
  • Analytic Sample Preparation Methods / methods*
  • Ellagic Acid / chemistry*
  • Fragaria / chemistry*
  • Fruit / chemistry
  • Hydrolysis
  • Hydrolyzable Tannins / chemistry*
  • Microwaves

Substances

  • Hydrolyzable Tannins
  • Ellagic Acid