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Methods Mol Biol. 2013;983:283-94. doi: 10.1007/978-1-62703-302-2_15.

Isolation of Dictyostelium nuclei for light and electron microscopy.

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  • 1Department of Cell Biology, Institute for Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany.


The significance of amoebae for studies of nuclear architecture has considerably increased in the recent years. The availability of a protocol for isolation of nuclei in a quality sufficient for high-resolution light and electron microscopy is a prerequisite for such studies. Here we present a protocol for high enrichment of nuclei by sucrose density-gradient centrifugation. Moreover, we describe how to use these isolated nuclei as specimens for immunofluorescence and immune-electron microscopy of ultrathin sections.

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