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Xenotransplantation. 2013 Mar-Apr;20(2):110-22. doi: 10.1111/xen.12026. Epub 2013 Mar 13.

Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.

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  • 1Stem Cell Center, Texas Heart Institute at St. Luke's Episcopal Hospital, Houston, 77030 TX, USA.

Abstract

BACKGROUND:

Stem cell therapy may help restore cardiac function after acute myocardial infarction (AMI), but the optimal therapeutic cell type has not been identified.

METHODS:

We examined the effects of CD34-/CD45- human unrestricted somatic stem cells (USSCs) in pigs (n = 30) with an AMI created by a 90-min occlusion of the left anterior descending coronary artery. Pigs were randomly assigned to receive either USSCs (302 ± 23 × 10(6) cells) or phosphate-buffered saline via 15 NOGA-guided transendocardial injections 10 days after AMI. Cyclosporine A (10 mg/kg orally, twice a day) was started in all pigs 3 days before control or cell treatment. Cardiac function was assessed by echocardiography before injection and at 4 and 8 weeks after treatment. Serum titers for pig IgG antibodies against USSCs were also measured at these time points and before AMI.

RESULTS:

Compared with control pigs, USSC-treated pigs showed no significant differences in any of the functional parameters examined. USSC-treated pigs showed variable increases in anti-USSC IgG antibody titers in the blood and chronic inflammatory infiltrates at the cell injection sites. Immunohistochemical studies of the injection sites using human anti-mitochondrial antibodies failed to detect implanted USSCs.

CONCLUSIONS:

We conclude that human USSCs did not improve cardiac function in a pig model of AMI. Cell transplantation in a xenogeneic setting may obscure the benefits of stem cell therapy.

© 2013 John Wiley & Sons A/S.

PMID:
23489741
[PubMed - indexed for MEDLINE]
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