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Iran J Microbiol. 2013 Mar;5(1):91-8.

A new approach for aggregation of Paramecium caudatum by nitric oxide.

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  • 1Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.

Abstract

BACKGROUND AND OBJECTIVES:

Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances.

MATERIALS AND METHODS:

A sedative drug, morphine, was infused into a cell chamber containing Paramecia. The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms.

RESULTS:

Morphine particularly (0.5 to 60 µg/µ1) aggregated the Paramecia. The infusion of L-arginine (1 to 8 µg/µ1) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µ1), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME.

CONCLUSION:

This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine.

KEYWORDS:

Aggregation; Morphine; NADPH-diaphorase; Nitric oxide; Paramecium

PMID:
23467065
[PubMed]
PMCID:
PMC3577564
Free PMC Article

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