Abstract
A cDNA library was constructed from an Angiostrongylus cantonensis young adult and the encoded proteins were expressed in Escherichia coli. One reactive antigen, a RAB-2 protein, was selected using an immunoscreening technique. The expression of the Th1-type cytokine IFN-γ was elicited in mouse splenic cells that were co-cultured with the recombinant RAB-2 protein and in the sera of mice that were immunised with this protein and adjuvant (50 μg at 2-week intervals). In the A. cantonensis-infected groups, the mice were orally infected with 35 infective larvae, and a subset of the infected mice were immunised with the recombinant RAB-2 protein in adjuvant. Serum samples were collected every week for ELISA, and the pathological examinations were performed at 14 days post infection (dpi). An increase in IFN-γ expression was noted in the blood, and the brain sections revealed moderate eosinophilic meningitis in the immunised mice. The RAB-2 antigen of A. cantonensis induced a Th1-type immune response both in vitro and in vivo.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiostrongylus cantonensis / genetics
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Angiostrongylus cantonensis / immunology*
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Animals
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Antigens, Helminth / genetics
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Antigens, Helminth / immunology*
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Biomphalaria
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Brain / parasitology
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Brain / pathology
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Cells, Cultured
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Helminth Proteins / genetics
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Helminth Proteins / immunology
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Interferon-gamma / blood
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Interferon-gamma / genetics
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Interferon-gamma / metabolism
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Interleukin-13 / genetics
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Interleukin-13 / metabolism
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Interleukin-4 / genetics
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Interleukin-4 / metabolism
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Interleukin-5 / genetics
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Interleukin-5 / metabolism
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Male
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Mice
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Mice, Inbred ICR
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Rabbits
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Random Allocation
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Rats
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Rats, Wistar
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Spleen / cytology
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Spleen / immunology
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Strongylida Infections / immunology*
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Strongylida Infections / parasitology
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Th1 Cells / immunology*
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rab2 GTP-Binding Protein / genetics
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rab2 GTP-Binding Protein / immunology*
Substances
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Antigens, Helminth
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Helminth Proteins
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Interleukin-13
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Interleukin-5
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Recombinant Proteins
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Interleukin-4
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Interferon-gamma
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rab2 GTP-Binding Protein