A RAB family antigen of Angiostrongylus cantonensis induces a Th1-type immune response in vitro and in vivo

Exp Parasitol. 2013 May;134(1):122-7. doi: 10.1016/j.exppara.2013.02.009. Epub 2013 Feb 28.

Abstract

A cDNA library was constructed from an Angiostrongylus cantonensis young adult and the encoded proteins were expressed in Escherichia coli. One reactive antigen, a RAB-2 protein, was selected using an immunoscreening technique. The expression of the Th1-type cytokine IFN-γ was elicited in mouse splenic cells that were co-cultured with the recombinant RAB-2 protein and in the sera of mice that were immunised with this protein and adjuvant (50 μg at 2-week intervals). In the A. cantonensis-infected groups, the mice were orally infected with 35 infective larvae, and a subset of the infected mice were immunised with the recombinant RAB-2 protein in adjuvant. Serum samples were collected every week for ELISA, and the pathological examinations were performed at 14 days post infection (dpi). An increase in IFN-γ expression was noted in the blood, and the brain sections revealed moderate eosinophilic meningitis in the immunised mice. The RAB-2 antigen of A. cantonensis induced a Th1-type immune response both in vitro and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiostrongylus cantonensis / genetics
  • Angiostrongylus cantonensis / immunology*
  • Animals
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology*
  • Biomphalaria
  • Brain / parasitology
  • Brain / pathology
  • Cells, Cultured
  • Helminth Proteins / genetics
  • Helminth Proteins / immunology
  • Interferon-gamma / blood
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism
  • Interleukin-13 / genetics
  • Interleukin-13 / metabolism
  • Interleukin-4 / genetics
  • Interleukin-4 / metabolism
  • Interleukin-5 / genetics
  • Interleukin-5 / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Rabbits
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Spleen / cytology
  • Spleen / immunology
  • Strongylida Infections / immunology*
  • Strongylida Infections / parasitology
  • Th1 Cells / immunology*
  • rab2 GTP-Binding Protein / genetics
  • rab2 GTP-Binding Protein / immunology*

Substances

  • Antigens, Helminth
  • Helminth Proteins
  • Interleukin-13
  • Interleukin-5
  • Recombinant Proteins
  • Interleukin-4
  • Interferon-gamma
  • rab2 GTP-Binding Protein