Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biosens Bioelectron. 2013 Jul 15;45:136-40. doi: 10.1016/j.bios.2013.01.044. Epub 2013 Feb 11.

Detection of target-probe oligonucleotide hybridization using synthetic nanopore resistive pulse sensing.

Author information

  • 1University of Auckland, Private Bag-92019, Auckland, New Zealand.

Abstract

Despite the plethora of DNA sensor platforms available, a portable, sensitive, selective and economic sensor able to rival current fluorescence-based techniques would find use in many applications. In this research, probe oligonucleotide-grafted particles are used to detect target DNA in solution through a resistive pulse nanopore detection technique. Using carbodiimide chemistry, functionalized probe DNA strands are attached to carboxylated dextran-based magnetic particles. Subsequent incubation with complementary target DNA yields a change in surface properties as the two DNA strands hybridize. Particle-by-particle analysis with resistive pulse sensing is performed to detect these changes. A variable pressure method allows identification of changes in the surface charge of particles. As proof-of-principle, we demonstrate that target hybridization is selectively detected at micromolar concentrations (nanomoles of target) using resistive pulse sensing, confirmed by fluorescence and phase analysis light scattering as complementary techniques. The advantages, feasibility and limitations of using resistive pulse sensing for sample analysis are discussed.

Copyright © 2013 Elsevier B.V. All rights reserved.

PMID:
23455053
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk