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Springerplus. 2013 Dec;2(1):43. doi: 10.1186/2193-1801-2-43. Epub 2013 Feb 9.

Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption.

Author information

  • 1Departamento de Microbiología, Universidad de Talca, Talca Camino Lircay s/n, Talca, Chile.

Abstract

The objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption. The virulence and bacteriocin genes of 78 E. faecalis were amplified by PCR and visualized in agarose gels. The antimicrobial susceptibility was determined through diffusion agar tests and the MIC through microdilution. It was observed that the major percentage of virulence genes in the E. faecalis strains corresponds to aggA (93.5%). The bacteriocin gene entA (64.1%) is the most frequently detected. The studied strains exhibited different virulence and bacteriocin genes, and an important antibacterial resistance. The most common resistant phenotype (n = 14) corresponds to tetracycline and chloramphenicol and the less frequent (n = 2) to ciprofloxacin and moxifloxacin. Eight different genetic profiles were observed for virulence y bacteriocin genes. It was determined a statistical association between the bacterial resistance and some of the genetic profiles detected.

KEYWORDS:

Antimicrobials; Bacteriocin; E. faecalis; Virulence; Water; Wells

PMID:
23449751
[PubMed]
PMCID:
PMC3579423
Free PMC Article
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