Methods for studies of protein interactions with different DNA methyltransferases

Jianchang Yang

doi:10.1007/978-1-62703-284-1_31

Methods for studies of protein interactions with different DNA methyltransferases

Methods Mol Biol. 2013:977:385-95. doi: 10.1007/978-1-62703-284-1_31.

Author

Jianchang Yang¹

Affiliation

¹ Human Health and Environment Program, Desert Research Institute (DRI), Las Vegas, NV, USA.

PMID: 23436379
DOI: 10.1007/978-1-62703-284-1_31

Abstract

There are now many methods available for studying protein interactions between DNA methltransferases (DNMTs) and their binding partners. Here we describe a step-by-step procedure to identify whether proteins of interest interact with DNMTs by co-immunoprecipitation (co-IP) assay in transiently transfected cells. Though one mammalian cell is described, investigators can use the same method with other cell lines or primary cells for in vivo protein interaction studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Fractionation
Chromatography, Affinity
DNA Modification Methylases / isolation & purification*
DNA Modification Methylases / metabolism
HEK293 Cells
Humans
Immunoprecipitation / methods
Protein Binding
Protein Interaction Mapping / methods*
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / isolation & purification
Transfection

Substances

Recombinant Fusion Proteins
DNA Modification Methylases