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Bioanalysis. 2013 Mar;5(5):603-12. doi: 10.4155/bio.13.13.

Quantitative imaging of a therapeutic peptide in biological tissue sections by MALDI MS.

Author information

  • 1Drug Metabolism & Pharmacokinetics, Drug Developmental Research Laboratories, Shionogi & Co. Ltd., 3-1-1 Futaba, Toyonaka, Osaka 561-0825, Japan. nozomi.takai@shionogi.co.jp

Abstract

BACKGROUND:

Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed.

RESULTS:

The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective.

CONCLUSION:

The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.

[PubMed - indexed for MEDLINE]
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